Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs.
Within this volume, a readers will find sections explaining general in addition to specific methods to the sample preparation. Numerous sections describe specific programs of AAA in clinical chemistry too as with food analysis, microbiology, marine biology, drug metabolic process, even archeology. Separate sections are dedicated to the use of AAA for protein quantitation and chiral AAA. Designed in the highly effective Techniques in Molecular Biology™ series format, sections contain introductions for their particular subjects, lists from the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and notes on troubleshooting and staying away from known issues.
English | ISBN: 1617794449 | 2011 | PDF | 377 pages | 5,73 mb
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